Anti-IKZF3 Antibody [ARC1755] (A307200)

Name: Anti-IKZF3 Antibody [ARC1755]
Brand: Antibodies.com
SKU: A307200

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This product is discontinued

Anti-IKZF3 Antibody [ARC1755] (A307200) has been discontinued and is no longer available.

View all Anti-IKZF3 Antibodies.

Specifications

Name

Anti-IKZF3 Antibody [ARC1755]

Description

Rabbit monoclonal [ARC1755] antibody to IKZF3.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human IKZF3 (Q9UKT9).

Sequence

MEDIQTNAELKSTQEQSVPAESAAVLNDYSLTKSHEMENVDSGEGPANEDEDIGDDSMKVKDEYSERDENVLKSEPMGNAEEPEIPYSYSREYNEYENIK

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1755

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

50 - 70 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AIO, Aiolos, IKAROS family zinc finger 3, IKAROS family zinc finger 3 (Aiolos), Ikaros family zinc finger protein 3, IKZF 3, IKZF3_HUMAN, zinc finger DNA binding protein Aiolos, Zinc finger protein Aiolos, Zinc finger protein subfamily 1A 3, Zinc finger protein subfamily 1A 3 (Aiolos), Zinc finger protein subfamily 1A, member 3, ZNFN1A3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-IKZF3 Antibody [ARC1755] (A307200)

Western blot analysis of Rat lung, using Anti-IKZF3 Antibody [ARC1755] (A307200) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 2: Western Blot - Anti-IKZF3 Antibody [ARC1755] (A307200)

Western blot analysis of Jurkat, using Anti-IKZF3 Antibody [ARC1755] (A307200) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 3: Immunohistochemistry - Anti-IKZF3 Antibody [ARC1755] (A307200)

Immunohistochemistry analysis of paraffin-embedded human appendix tissue using Anti-IKZF3 Antibody [ARC1755] (A307200) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-IKZF3 Antibody [ARC1755] (A307200)

Immunofluorescence analysis of rat spleen using Anti-IKZF3 Antibody [ARC1755] (A307200) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).