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Anti-Interferon gamma Antibody (A88828)

Western Blot - Anti-Interferon gamma Antibody (A88828) - Antibodies.com Immunohistochemistry - Anti-Interferon gamma Antibody (A88828) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to Interferon gamma for WB and IHC.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
Anti-Interferon gamma Antibody
Description
Rabbit polyclonal antibody to Interferon gamma.
Applications
WB, IHC
Dilutions
WB: 1:2,000-1:10,000, IHC: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 23-161 of IFN gamma (NP_000610.2).
Sequence
CQDPYVKEAENLKKYFNAGHSDVADNGTLFLGILKNWKEESDRKIMQSQIVSFYFKLFKNFKDDQSIQKSVETIKEDMNVKFFNSNKKKRDDFEKLTNYSVTDLNVQRKAIHELIQVMAELSPAAKTGKRKRSQMLFRG
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
17 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
IFG , IFI, IFN gamma, IFN Immune , IFN, immune, IFN-gamma, IFNG, IFNG_HUMAN, Immune interferon, Interferon Gamma Precursor, Macrophage Activating Factor , MAF, T Cell Interferon , Type II Interferon
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Interferon gamma Antibody (A88828) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Interferon gamma Antibody (A88828)
Western blot analysis of Recombinant Human IFN-gamma Protein, using Anti-Interferon gamma Antibody (A88828) at 1:9000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry - Anti-Interferon gamma Antibody (A88828) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-Interferon gamma Antibody (A88828)
Immunohistochemistry analysis of paraffin-embedded human lung using Anti-Interferon gamma Antibody (A88828) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Publishing research using Anti-Interferon gamma Antibody (A88828)? Please let us know so that we can list the citation on this page.

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