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Anti-Interferon gamma Antibody [ARC57549] (A305530)

Western Blot - Anti-Interferon gamma Antibody [ARC57549] (A305530) - Antibodies.com Western Blot - Anti-Interferon gamma Antibody [ARC57549] (A305530) - Antibodies.com
$470
Product Datasheet

Rabbit monoclonal [ARC57549] antibody to Interferon gamma for WB.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-Interferon gamma Antibody [ARC57549]
Description
Rabbit monoclonal [ARC57549] antibody to Interferon gamma.
Applications
WB
Dilutions
WB: 1:500-1:1,000
Reactivity
Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 20-153 of rat IFN gamma (NP_620235.1).
Sequence
CYCQGTLIESLESLKNYFNSSSMDAMEGKSLLLDIWRNWQKDGNTKILESQIISFYLRLFEVLKDNQAISNNISVIESHLITNFFSNSKAkDaFMSIAKFEVNNPQIQHKAVNELIRVIHQLSPESSLRKRKR
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC57549
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
50 - 55 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
IFG , IFI, IFN gamma, IFN Immune , IFN, immune, IFN-gamma, IFNG, IFNG_HUMAN, Immune interferon, Interferon Gamma Precursor, Macrophage Activating Factor , MAF, T Cell Interferon , Type II Interferon
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Interferon gamma Antibody [ARC57549] (A305530) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Interferon gamma Antibody [ARC57549] (A305530)
Western blot analysis of Recombinant Rat IFN-gamma Protein, using Anti-Interferon gamma Antibody [ARC57549] (A305530) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 120s.
Western Blot - Anti-Interferon gamma Antibody [ARC57549] (A305530) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-Interferon gamma Antibody [ARC57549] (A305530)
Western blot analysis of Recombinant Mouse IFN-gamma Protein, using Anti-Interferon gamma Antibody [ARC57549] (A305530) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 120s.
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