Anti-IRE1 Antibody (A92812)

Name: Anti-IRE1 Antibody
Brand: Antibodies.com
SKU: A92812
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to IRE1 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-IRE1 Antibody

Description

Rabbit polyclonal antibody to IRE1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S724 of human IRE1 (NP_001424.3).

Sequence

RHSFS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

130 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Endoplasmic reticulum (ER) to nucleus signalling 1, Endoplasmic reticulum to nucleus signaling 1, Endoplasmic reticulum-to-nucleus signaling 1, Endoribonuclease, ER to nucleus signaling 1, ERN 1, Ern1, ERN1_HUMAN, hIRE 1p, hIRE1p, Inositol requiring 1, Inositol requiring 1, S. cerevisiae, homolog of, Inositol requiring enzyme 1, S. cerevisiae, homolog of, Inositol requiring protein 1, inositol-requiring enzyme 1, Inositol-requiring protein 1, IRE 1, IRE 1a, IRE 1P, Ire1 alpha, Ire1-alpha, IRE1a, Ire1alpha, IRE1P, MGC163277, MGC163279, Protein kinase/endoribonuclease, RGD1559716, Serine/threonine protein kinase/endoribonuclease IRE1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (10)

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Figure 1: Western Blot - Anti-IRE1 Antibody (A92812)

Western blot analysis of extracts of 293T cells, using Anti-IRE1 Antibody (A92812) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 120s.

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Figure 2: Western Blot - Anti-IRE1 Antibody (A92812)

Western blot analysis of extracts of various cell lines, using Anti-IRE1 Antibody (A92812) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 3: Immunohistochemistry - Anti-IRE1 Antibody (A92812)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-IRE1 Antibody (A92812) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-IRE1 Antibody (A92812)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-IRE1 Antibody (A92812) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.