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Anti-IRF3 Antibody (A80489)

Western Blot - Anti-IRF3 Antibody (A11118) - Antibodies.com Western Blot - Anti-IRF3 Antibody (A11118) - Antibodies.com Immunohistochemistry - Anti-IRF3 Antibody (A11118) - Antibodies.com Immunohistochemistry - Anti-IRF3 Antibody (A11118) - Antibodies.com Chromatin Immunoprecipitation - Anti-IRF3 Antibody (A11118) - Antibodies.com
This product is discontinued

Anti-IRF3 Antibody (A80489) has been discontinued and is no longer available.

View all Anti-IRF3 Antibodies.

Name
Anti-IRF3 Antibody
Description
Rabbit polyclonal antibody to IRF3.
Applications
WB, IHC, IF, IP, ChIP
Dilutions
WB: 1:500-1:2000, IHC: 1:50-1:200, IF: 1:50-1:200, ChIP:: 1:20-1:100
Reactivity
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human IRF3 (NP_001184051.1).
Sequence
MGTPKPRILPWLVSQLDLGQLEGVAWVNKSRTRFRIPWKHGLRQDAQQEDFGIFQAWAEATGAYVPGRDKPDLPTWKRNFRSALNRKEGLRLAEDRSKDPHDPHKIYEFVNSGVGDFSQPDTSPDTNGGGSTSDTQEDILDELLGNMVLAPLPDPGPPSLAVAPEPCPQPLRSPSLDNPTPFPNLGPSENPLKRLLVPGEEWEFEVTAFYRGRQVFQQTISCPEGLRLVGSEVGDRTLPGWPVTLPDPGMSLTDRGVMSY
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
56kDa
Formulation
Supplied in Phosphate Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
IIAE7, Interferon regulatory factor 3, IRF 3, IRF-3, IRF3_HUMAN, MGC94729
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-IRF3 Antibody (A11118) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-IRF3 Antibody (A80489)
Western blot analysis of extracts of various cell lines, using Anti-IRF3 Antibody (A11118) at 1:3000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Western Blot - Anti-IRF3 Antibody (A11118) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-IRF3 Antibody (A80489)
Western blot analysis of extracts from normal (control) and IRF3 knockout (KO) HeLa cells, using Anti-IRF3 Antibody (A11118) at 1:3000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
Immunohistochemistry - Anti-IRF3 Antibody (A11118) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-IRF3 Antibody (A80489)
Immunohistochemistry of paraffin-embedded human lung cancer using Anti-IRF3 Antibody (A11118) at dilution of 1:100 (40x lens).
Immunohistochemistry - Anti-IRF3 Antibody (A11118) - Antibodies.comEnlarge Image
Figure 4: Immunohistochemistry - Anti-IRF3 Antibody (A80489)
Immunohistochemistry of paraffin-embedded human esophageal cancer using Anti-IRF3 Antibody (A11118) at dilution of 1:100 (40x lens).
Publishing research using Anti-IRF3 Antibody (A80489)? Please let us know so that we can list the citation on this page.

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