Anti-ISG15 Antibody [ARC53794] (A307409)

Name: Anti-ISG15 Antibody [ARC53794]
Brand: Antibodies.com
SKU: A307409
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC53794] antibody to ISG15 for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-ISG15 Antibody [ARC53794]

Description

Rabbit monoclonal [ARC53794] antibody to ISG15.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human ISG15 (NP_005092.1).

Sequence

MGWDLTVKMLAGNEFQVSLSSSMSVSELKAQITQKIGVHAFQQRLAVHPSGVALQDRVPLASQGLGPGSTVLLVVDKCDEPLSILVRNNKGRSSTYEVRLTQTVAHLKQQVSGLEGVQDDLFWLTFEGKPLEDQLPLGEYGLKPLSTVFMNLRLRGGGTEPGGRS

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC53794

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

15 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

G1P2, hUCRP, IFI 15, IFI15, Interferon alpha inducible protein, Interferon induced protein 15, Interferon induced protein IFI-15K, Interferon stimulated protein 15 kDa, Interferon, alpha-inducible protein (clone IFI-15K), Interferon-induced 15 kDa protein, Interferon-induced 17 kDa protein, Interferon-induced 17-kDa/15-kDa protein, IP17, ISG 15, ISG15 ubiquitin like modifier, ISG15_HUMAN, Ubiquitin cross-reactive protein, Ubiquitin-like protein ISG15, UCRP

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-ISG15 Antibody [ARC53794] (A307409)

Western blot analysis of extracts of various cell lines, using Anti-ISG15 Antibody [ARC53794] (A307409) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Figure 2: Immunofluorescence - Anti-ISG15 Antibody [ARC53794] (A307409)

Immunofluorescence analysis of C6 cells using Anti-ISG15 Antibody [ARC53794] (A307409) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 3: Immunofluorescence - Anti-ISG15 Antibody [ARC53794] (A307409)

Immunofluorescence analysis of NIH-3T3 cells using Anti-ISG15 Antibody [ARC53794] (A307409) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-ISG15 Antibody [ARC53794] (A307409)

Immunofluorescence analysis of U-2 OS cells using Anti-ISG15 Antibody [ARC53794] (A307409) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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