Anti-Ku80 Antibody (A9759)

Name: Anti-Ku80 Antibody
Brand: Antibodies.com
SKU: A9759

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This product is discontinued

Anti-Ku80 Antibody (A9759) has been discontinued and is no longer available.

View all Anti-Ku80 Antibodies.

Specifications

Name

Anti-Ku80 Antibody

Description

Rabbit polyclonal antibody to Ku80.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:100-1:200, ICC/IF: 1:20-1:100, IP: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 543-732 of human Ku80 (NP_066964.1).

Sequence

KKKDQVTAQEIFQDNHEDGPTAKKLKTEQGGAHFSVSSLAEGSVTSVGSVNPAENFRVLVKQKKASFEEASNQLINHIEQFLDTNETPYFMKSIDCIRAFREEAIKFSEEQRFNNFLKALQEKVEIKQLNHFWEIVVQDGITLITKEEASGSSVTAEEAKKFLAPKDKPSGDTAAVFEEGGDVDDLLDMI

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

86 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

86 kDa subunit of Ku antigen, ATP dependent DNA helicase 2 subunit 2, ATP dependent DNA helicase II, ATP dependent DNA helicase II 80 kDa subunit, ATP dependent DNA helicase II 86 Kd subunit, ATP-dependent DNA helicase 2 subunit 2, ATP-dependent DNA helicase II 80 kDa subunit, CTC box binding factor 85 kDa, CTC box-binding factor 85 kDa subunit, CTC85, CTCBF, DNA repair protein XRCC5, KARP 1, KARP1, Ku 80, Ku autoantigen 80kDa, Ku86, Ku86 autoantigen related protein 1, KUB 2, KUB2, Lupus Ku autoantigen protein p86, NFIV, Nuclear factor IV, Thyroid lupus autoantigen, Thyroid-lupus autoantigen, TLAA, X ray repair complementing defective repair in Chinese hamster cells 5 (double strand break rejoining), X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining), X-ray repair cross-complementing protein 5, Xray repair complementing defective repair in Chinese hamster cells 5, XRCC 5, XRCC5, XRCC5_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (7)

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Figure 1: Western Blot - Anti-Ku80 Antibody (A9759)

Western blot analysis of extracts of various cell lines, using Anti-Ku80 Antibody (A9759) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunohistochemistry - Anti-Ku80 Antibody (A9759)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-Ku80 Antibody (A9759) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Ku80 Antibody (A9759)

Immunohistochemistry analysis of paraffin-embedded human gastric cancer using Anti-Ku80 Antibody (A9759) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.