Anti-L1CAM Antibody [ARC57419] (A309655)

Name: Anti-L1CAM Antibody [ARC57419]
Brand: Antibodies.com
SKU: A309655

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This product is discontinued

Anti-L1CAM Antibody [ARC57419] (A309655) has been discontinued and is no longer available.

View all Anti-L1CAM Antibodies.

Specifications

Name

Anti-L1CAM Antibody [ARC57419]

Description

Rabbit monoclonal [ARC57419] antibody to L1CAM.

Applications

WB, IHC

Dilutions

WB: 1:2,000-1:9,000, IHC: 1:100-1:500

Reactivity

Human

Immunogen

Recombinant protein of human CD171/L1CAM.

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC57419

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Antigen identified by monoclonal, CAML1, CD171, CD171 antigen, HSAS, HSAS1, Hyd, L1, L1 cell adhesion molecule, L1-NCAM, L1CAM_HUMAN, MASA, MIC5, N CAML1, N-CAM-L1, NCAM-L1, NCAML1, Nerve-growth factor-inducible large external glycoprotein, Neural cell adhesion molecule L1, NILE, OTTHUMP00000025992, S10, SPG1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-L1CAM Antibody [ARC57419] (A309655)

Western blot analysis of various lysates, using Anti-L1CAM Antibody [ARC57419] (A309655) at 1:8,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Immunohistochemistry - Anti-L1CAM Antibody [ARC57419] (A309655)

Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-L1CAM Antibody [ARC57419] (A309655) at a dilution of 1:400 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-L1CAM Antibody [ARC57419] (A309655)

Immunohistochemistry analysis of paraffin-embedded human kidney using Anti-L1CAM Antibody [ARC57419] (A309655) at a dilution of 1:400 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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