Anti-Lamin B1 Antibody [ARC0621] (A81050)

Name: Anti-Lamin B1 Antibody [ARC0621]
Brand: Antibodies.com
SKU: A81050

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This product is discontinued

Anti-Lamin B1 Antibody [ARC0621] (A81050) has been discontinued and is no longer available.

View all Anti-Lamin B1 Antibodies.

Highlighted products for Anti-Lamin B1 Antibody

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A82838

Anti-Lamin B1 Antibody

Goat polyclonal antibody to Lamin B1 for ELISA and WB.

(2)

A82838

Anti-Lamin B1 Antibody

Goat polyclonal antibody to Lamin B1 for ELISA and WB.

Specifications

Name

Anti-Lamin B1 Antibody [ARC0621]

Description

Rabbit monoclonal [ARC0621] antibody to Lamin B1.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 450-550 of human Lamin B1 (P20700).

Sequence

DGKFIRLKNTSEQDQPMGGWEMIRKIGDTSVSYKYTSRYVLKAGQTVTIWAANAGVTASPPTDLIWKNQNSWGTGEDVKVILKNSQGEEVAQRSTVFKTTI

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0621

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

45 kDa / 70 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ADLD, Lamin-B1, LMN, LMN2, LMNB, Lmnb1, LMNB1_HUMAN, MGC111419, OTTHUMP00000159218

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Lamin B1 Antibody [ARC0621] (A81050)

Western blot analysis of extracts of various cell lines, using Anti-Lamin B1 Antibody [ARC0621] (A81050) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunohistochemistry - Anti-Lamin B1 Antibody [ARC0621] (A81050)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-Lamin B1 Antibody [ARC0621] (A81050) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Lamin B1 Antibody [ARC0621] (A81050)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-Lamin B1 Antibody [ARC0621] (A81050) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Lamin B1 Antibody [ARC0621] (A81050)

Immunohistochemistry analysis of paraffin-embedded rat spleen using Anti-Lamin B1 Antibody [ARC0621] (A81050) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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