Anti-LRP4 Antibody [S207-27] (A304980)

Name: Anti-LRP4 Antibody [S207-27]
Brand: Antibodies.com
SKU: A304980
Price: 500.0 USD
Availability: InStock

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$500

Mouse monoclonal [S207-27] antibody to LRP4 for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-LRP4 Antibody [S207-27]

Description

Mouse monoclonal [S207-27] antibody to LRP4.

Specificity

This antibody detects the extracellular domain of LRP4. Degradation product can be seen at ~150 kDa.

Applications

WB, ICC/IF

Dilutions

WB: 1:1,000, ICC/IF: 1:100

Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein amino acids 26- 350 (extracellular N-terminus) of mouse LTP4 .

Host

Mouse

Clonality

Monoclonal

Clone ID

S207-27

Isotype

IgG2a

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~215 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

KIAA0816, LDLR dan, Low density lipoprotein receptor related protein 4, Low-density lipoprotein receptor-related protein 4, LRP-4, LRP10, LRP4_HUMAN, MEGF7, Multiple epidermal growth factor like domains 7, Multiple epidermal growth factor-like domains 7

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Immunofluorescence - Anti-LRP4 Antibody [S207-27] (A304980)

Immunocytochemistry/Immunofluorescence analysis of human COS cells transfected with 2 ug human LRP4 plasmid, fixed in 4% PFA for 10 min, using Anti-LRP4 Antibody [S207-27] (A304980), at 1:100 for 1 hour at room temperature. The secondary antibody used was Goat anti-mouse: Alexa 488. Counterstain: DAPI.

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Figure 2: Western Blot - Anti-LRP4 Antibody [S207-27] (A304980)

Western blot analysis of rat brain membrane lysate showing detection of LRP4 protein using Anti-LRP4 Antibody [S207-27] (A304980) at 1:500, 1:1000, and 1:2000.

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Figure 3: Immunofluorescence - Anti-LRP4 Antibody [S207-27] (A304980)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-LRP4 Antibody [S207-27] (A304980), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60min room temperature, 5min room temperature. Localization: Membrane. Magnification: 60X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) LRP4 (Extracellular) Antibody. (D) Composite.

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Figure 4: Immunofluorescence - Anti-LRP4 Antibody [S207-27] (A304980)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-LRP4 Antibody [S207-27] (A304980), at 1:200 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) LRP4 (Extracellular) Antibody (D) Composite.

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