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Anti-MAP2K1 (phospho S221) Antibody (A16500)

Western Blot - Anti-MAP2K1 (phospho S221) Antibody (AP0188) - Antibodies.com Immunohistochemistry - Anti-MAP2K1 (phospho S221) Antibody (AP0188) - Antibodies.com Immunofluorescence - Anti-MAP2K1 (phospho S221) Antibody (AP0188) - Antibodies.com
This product is discontinued

Anti-MAP2K1 (phospho S221) Antibody (A16500) has been discontinued and is no longer available.

View all MAP2K1 Antibodies.

  • Specifications

Name
Anti-MAP2K1 (phospho S221) Antibody
Description
Rabbit polyclonal antibody to MAP2K1 (phospho S221).
Applications
WB, IHC, IF
Dilutions
WB: 1:500-1:2000, IHC: 1:50-1:100, IF: 1:100-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A phospho specific peptide corresponding to residues surrounding S221 of human MAP2K1.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
43kDa
Formulation
Supplied in Phosphate Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAP kinase kinase 1, MAPK/ERK kinase 1, MAPKK 1, MEK 1, MKK1, PRKMK1
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-MAP2K1 (phospho S221) Antibody (AP0188) - Antibodies.comEnlarge Image

Western Blot - Anti-MAP2K1 (phospho S221) Antibody (A16500)

Western blot analysis of extracts from HeLa cells, using Anti-MAP2K1 (phospho S221) Antibody (AP0188). Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% BSA.
Immunohistochemistry - Anti-MAP2K1 (phospho S221) Antibody (AP0188) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-MAP2K1 (phospho S221) Antibody (A16500)

Immunohistochemistry of paraffin-embedded human breast carcinoma using Anti-MAP2K1 (phospho S221) Antibody (AP0188).
Immunofluorescence - Anti-MAP2K1 (phospho S221) Antibody (AP0188) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-MAP2K1 (phospho S221) Antibody (A16500)

Immunofluorescence analysis of methanol-fixed HeLa cells using Anti-MAP2K1 (phospho S221) Antibody (AP0188).

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Proteins predicted to interact with MEK1

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.8% Relevancy Score
99.7% Relevancy Score
99.6% Relevancy Score
99.6% Relevancy Score
99.5% Relevancy Score