Supplied as an aliquot of IgY preparation with 5mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
General Notes
This antibody can be used to verify the size of fusion constructs by western blotting, and to amplify the endogenous fluorescence of mCherry in transfected cells.
HEK293 cells transfected in the same way and viewed in the confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with Cherry are bright red. Staining with Anti-mCherry Antibody is shown in Green. Green antibody staining is only seen in cells which express Cherry, as expected, and the superimposition of the green and red results in an orange signal. Interestingly stronger Cherry staining is seen in the nucleus, possibly due to degradation of some Cherry molecules to release the low molecular weight Cherry fluorochrome.
Immunofluorescent analysis of HEK293 cells transfected with mCherry and stained with Anti-mCherry Antibody (A85304), viewed in a confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are bright red, and staining with Anti-mCherry Antibody (A85304) is shown in Green. The green antibody staining is only seen cells which express mCherry, as expected, and the superimposition of the green and red results in an orange signal. Interestingly stronger mCherry staining is seen in the nucleus, possibly due to degradation of some mCherry molecules releasing the low molecular weight mCherry fluorochrome.
Figure 3: Western Blot - Anti-mCherry Antibody (A85304)
Western blot analysis of Anti-mCherry Antibody (1:2,000): 1: HEK293 cells transfected with pFin-EF1-mCherry vector. There is a strong clean band at about 29 kDa corresponding to mCherry. 2: HEK293 cells which were not transfected with this vector show no protein bands.
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