Anti-Melanoma gp100 Antibody [SPM142] (A249968)

Mouse monoclonal [SPM142] antibody to Melanoma gp100 for Flow Cytometry, IF, WB and IHC-P.

Alternative Formats and ConjugatesAlternative Formats

Freight Charges
Lead Time
4-6 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Anti-Melanoma gp100 Antibody [SPM142]
Description
Mouse monoclonal [SPM142] antibody to Melanoma gp100.
Specificity
The gp100 molecule is a 100kDa glycosylated protein that is cleaved into a small (26kDa) carboxy-terminal fragment and a larger amino- terminal section (60-64 kDa), which is subsequently cleaved to generate 26kDa and 34-38kDa fragments. By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. It stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This MAb also stains Angiomyolipoma.
Applications
Flow Cytometry, IF, WB, IHC-P
Dilutions
Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, WB: 2-4 µg/ml, IHC-P: 1-2 µg/ml
Reactivity
Human
Cross Reactivity
This antibody does not cross react with Rat or Canine.
Immunogen
Extract of pigmented melanoma metastases from lymph nodes.
Host
Mouse
Clonality
Monoclonal
Clone ID
SPM142
Isotype
IgG1
Light Chains
kappa
Conjugate

Unconjugated

Purification
Protein A/G chromatography.
Concentration
200 µg/ml
Molecular Weight
26-100 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
General Notes
This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-Melanoma gp100 Antibody [SPM142] - BSA and Azide free (A253148).
Synonyms
95 kDa melanocyte specific secreted glycoprotein, 95 kDa melanocyte-specific secreted glycoprotein, D12S53E, gp100, M-beta, ME20, ME20 M/ME20 S, ME20-M, ME20-S, ME20M, ME20M/ME20S, ME20S, Melanocyte lineage specific antigen GP100, Melanocyte protein mel 17, Melanocyte protein Pmel 17, Melanocyte protein Pmel 17 precursor, Melanocytes lineage-specific antigen GP100, Melanoma associated ME20 antigen, Melanoma-associated ME20 antigen, Melanosomal matrix protein 17, Melanosomal matrix protein17, P1, p100, p26, PMEL, PMEL 17, PMEL17, PMEL_HUMAN, Premelanosome protein, Secreted melanoma-associated ME20 antigen, SI, SIL, SILV, Silver (mouse homolog) like, Silver homolog, Silver locus protein homolog, Silver, mouse, homolog of
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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