Anti-METTL3 Antibody [ARC0487] (A308621)

Name: Anti-METTL3 Antibody [ARC0487]
Brand: Antibodies.com
SKU: A308621
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0487] antibody to METTL3 for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-METTL3 Antibody [ARC0487]

Description

Rabbit monoclonal [ARC0487] antibody to METTL3.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 2-250 of human METTL3 (Q86U44).

Sequence

SDTWSSIQAHKKQLDSLRERLQRRRKQDSGHLDLRNPEAALSPTFRSDSPVPTAPTSGGPKPSTASAVPELATDPELEKKLLHHLSDLALTLPTDAVSICLAISTPDAPATQDGVESLLQKFAAQELIEVKRGLLQDDAHPTLVTYADHSKLSAMMGAVAEKKGPGEVAGTVTGQKRRAEQDSTTVAAFASSLVSGLNSSASEPAKEPAKKSRKHAASDVDLEIESLLNQQSTKEQQSKKVSQEILELL

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0487

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

80 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

adoMet-binding subunit of the human mRNA (N6-adenosine)-methyltransferase, IME4, M6A, Methyltransferase like protein 3, Methyltransferase-like protein 3, mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase, mRNA m(6)A methyltransferase, MT-A70, MTA70, MTA70_HUMAN, N6 adenosine methyltransferase 70 kDa subunit, N6-adenosine-methyltransferase 70 kDa subunit

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-METTL3 Antibody [ARC0487] (A308621)

Western blot analysis of extracts of various cell lines, using Anti-METTL3 Antibody [ARC0487] (A308621) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.

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Figure 2: Immunofluorescence - Anti-METTL3 Antibody [ARC0487] (A308621)

Immunofluorescence analysis of HeLa cells using Anti-METTL3 Antibody [ARC0487] (A308621) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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