Anti-MiTF Antibody [ARC55801] (A306180)

Name: Anti-MiTF Antibody [ARC55801]
Brand: Antibodies.com
SKU: A306180

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This product is discontinued

Anti-MiTF Antibody [ARC55801] (A306180) has been discontinued and is no longer available.

View all Anti-MiTF Antibodies.

Specifications

Name

Anti-MiTF Antibody [ARC55801]

Description

Rabbit monoclonal [ARC55801] antibody to MiTF.

Applications

WB, ICC/IF

Dilutions

WB: 1:2,000-1:20,000, ICC/IF: 1:100-1:500

Reactivity

Human, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 402-526 of human MITF (NP_001341533.1).

Sequence

HGLSLIPSTGLCSPDLVNRIIKQEPVLENCSQDLLQHHADLTCTTTLDLTDGTITFNNNLGTGTEANQAYSVPTKMGSKLEDILMDDTLSPVGVTDPLLSSVSPGASKTSSRRSSMSMEETEHTC

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC55801

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

50 - 75 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

bHLHe32, Class E basic helix-loop-helix protein 32, CMM8, Homolog of mouse microphthalmia, Mi, Microphthalmia associated transcription factor, Microphthalmia, mouse, homolog of, Microphthalmia-associated transcription factor, mitfa, MITF_HUMAN, nacre, WS2, WS2A, z3A.1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-MiTF Antibody [ARC55801] (A306180)

Western blot analysis of extracts of Hela cells, using Anti-MiTF Antibody [ARC55801] (A306180) at 1:20,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Western Blot - Anti-MiTF Antibody [ARC55801] (A306180)

Western blot analysis of extracts of Rat heart, using Anti-MiTF Antibody [ARC55801] (A306180) at 1:20,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

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Figure 3: Immunofluorescence - Anti-MiTF Antibody [ARC55801] (A306180)

Immunofluorescence analysis of HeLa using Anti-MiTF Antibody [ARC55801] (A306180) at a dilution of 1:300 (40x lens). DAPI was used to stain the cell nuclei (blue).

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