Anti-MMP13 Antibody [ARC0528] (A308452)

Name: Anti-MMP13 Antibody [ARC0528]
Brand: Antibodies.com
SKU: A308452

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This product is discontinued

Anti-MMP13 Antibody [ARC0528] (A308452) has been discontinued and is no longer available.

View all Anti-MMP13 Antibodies.

Specifications

Name

Anti-MMP13 Antibody [ARC0528]

Description

Rabbit monoclonal [ARC0528] antibody to MMP13.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 350-450 of human MMP13 (P45452).

Sequence

RGRKFWALNGYDILEGYPKKISELGLPKEVKKISAAVHFEDTGKTLLFSGNQVWRYDDTNHIMDKDYPRLIEEDFPGIGDKVDAVYEKNGYIYFFNGPIQF

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0528

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

60 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CLG 3, CLG3, Collagenase 3, Collagenase3, MANDP1, Matrix metallopeptidase 13 (collagenase 3), Matrix Metalloproteinase 13, Matrix metalloproteinase-13, MMP 13, MMP-13, MMP13_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-MMP13 Antibody [ARC0528] (A308452)

Western blot analysis of extracts of various cell lines, using Anti-MMP13 Antibody [ARC0528] (A308452) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Immunohistochemistry - Anti-MMP13 Antibody [ARC0528] (A308452)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-MMP13 Antibody [ARC0528] (A308452) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-MMP13 Antibody [ARC0528] (A308452)

Confocal imaging of U-2 OS cells using Anti-MMP13 Antibody [ARC0528] (A308452), at a dilution of 1:100, (red). The cells were counterstained with Anti-alpha Tubulin Antibody, at a dilution of 1:400, (green). DAPI was used for nuclear staining (Blue). Objective: 60x.

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