Anti-MSH2 Antibody (A307086)

Name: Anti-MSH2 Antibody
Brand: Antibodies.com
SKU: A307086
Price: 470.0 USD
Availability: InStock

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$470

Rabbit polyclonal antibody to MSH2 for WB, ICC/IF and IP.

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Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Knockout Validated Antibody

Specifications

Name

Anti-MSH2 Antibody

Description

Rabbit polyclonal antibody to MSH2.

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200, IP: 1:1,000-1:5,000

Reactivity

Human, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human MSH2 (NP_000242.1).

Sequence

MAVQPKETLQLESAAEVGFVRFFQGMPEKPTTTVRLFDRGDFYTAHGEDALLAAREVFKTQGVIKYMGPAGAKNLQSVVLSKMNFESFVKDLLLVRQYRVEVYKNRAGNKASKENDWYLAYKASPGNLSQFEDILFGNNDMSASIGVVGVKMSAVDGQRQVGVGYVDSIQRKLGLCEFPDNDQFSNLEALLIQIGPKECVLPGGETAGDMGKLRQIIQRGGILITERKKADFSTKDIYQDLNRLLKGKKGEQMNSAVLPEMENQVAVSSLSAVIKFLELLSDDSNFGQFELTTFDFSQYM

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

105 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

BAT26, COCA 1, COCA1, DNA mismatch repair protein Msh2, FCC 1, FCC1, hMSH2, HNPCC, HNPCC 1, HNPCC1, LCFS2, MSH 2, MSH2_HUMAN, MutS homolog 2, MutS homolog 2 colon cancer nonpolyposis type 1, MutS protein homolog 2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-MSH2 Antibody (A307086)

Western blot analysis of A-549, using Anti-MSH2 Antibody (A307086) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-MSH2 Antibody (A307086)

Western blot analysis of extracts from wild type(WT) and MSH2 Rabbit polyclonal antibody knockout (KO) HeLa cells, using Anti-MSH2 Antibody (A307086) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 3: Western Blot - Anti-MSH2 Antibody (A307086)

Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-MSH2 Antibody (A307086). This Western blot was performed on the immunoprecipitate using Anti-MSH2 Antibody (A307086) at a dilution of 1:3000.

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