Figure 1: Western Blot - Anti-MSH2 Antibody [ARC55799] (A306655)
Western blot analysis of extracts from wild type(WT) and MSH2 knockout (KO) HeLa cells, using Anti-MSH2 Antibody [ARC55799] (A306655) at 1:20,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Figure 2: Western Blot - Anti-MSH2 Antibody [ARC55799] (A306655)
Western blot analysis of various lysates, using Anti-MSH2 Antibody [ARC55799] (A306655) at 1:20,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded human appendix tissue using Anti-MSH2 Antibody [ARC55799] (A306655) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-MSH2 Antibody [ARC55799] (A306655) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human colon cancer loss of MSH2 expression using Anti-MSH2 Antibody [ARC55799] (A306655) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.