Anti-MSH6 Antibody [ARC57727] (A309410)

Name: Anti-MSH6 Antibody [ARC57727]
Brand: Antibodies.com
SKU: A309410
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC57727] antibody to MSH6 for WB, IHC and ICC/IF.

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Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Tags:

Knockout Validated Antibody

Specifications

Name

Anti-MSH6 Antibody [ARC57727]

Description

Rabbit monoclonal [ARC57727] antibody to MSH6.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:1,000-1:5,000, IHC: 1:100-1:500, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human MSH6.

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC57727

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

DNA mismatch repair protein Msh6, G/T mismatch binding protein, G/T mismatch-binding protein, GTBP, GTMBP, hMSH6, HNPCC 5, HNPCC5, HSAP, MSH 6, MSH6_HUMAN, mutS (E. coli) homolog 6, MutS alpha 160 kDa subunit, mutS homolog 6, MutS homolog 6 (E. coli), MutS-alpha 160 kDa subunit, p160, Sperm associated protein

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-MSH6 Antibody [ARC57727] (A309410)

Western blot analysis of various lysates, using Anti-MSH6 Antibody [ARC57727] (A309410) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Figure 2: Western Blot - Anti-MSH6 Antibody [ARC57727] (A309410)

Western blot analysis of Rat testis, using Anti-MSH6 Antibody [ARC57727] (A309410) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Figure 3: Western Blot - Anti-MSH6 Antibody [ARC57727] (A309410)

Western blot analysis of extracts from wild type(WT) and MSH6 knockout (KO) 293T cells, using Anti-MSH6 Antibody [ARC57727] (A309410) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Figure 4: Immunohistochemistry - Anti-MSH6 Antibody [ARC57727] (A309410)

Immunohistochemistry analysis of paraffin-embedded human appendix tissue using Anti-MSH6 Antibody [ARC57727] (A309410) at a dilution of 1:400 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.