Anti-MTAP Antibody [ARC2872] (A305896)

Name: Anti-MTAP Antibody [ARC2872]
Brand: Antibodies.com
SKU: A305896

Enlarge Image

This product is discontinued

Anti-MTAP Antibody [ARC2872] (A305896) has been discontinued and is no longer available.

View all Anti-MTAP Antibodies.

Specifications

Name

Anti-MTAP Antibody [ARC2872]

Description

Rabbit monoclonal [ARC2872] antibody to MTAP.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 184-283 of human MTAP (Q13126).

Sequence

FMFRTWGADVINMTTVPEVVLAKEAGICYASIAMATDYDCWKEHEEAVSVDRVLKTLKENANKAKSLLLTTIPQIGSTEWSETLHNLKNMAQFSVLLPRH

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2872

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

29 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

5' methylthioadenosine phosphorylase, 5''-methylthioadenosine phosphorylase, BDMF, c86fus, DMSFH, DMSMFH, Epididymis luminal protein 249, HEL 249, LGMBF, MeSAdo phosphorylase, Methylthioadenosine phosphorylase, MSAP, MTA phosphorylase, MTAPase, MTAP_HUMAN, S methyl 5 thioadenosine phosphorylase, S methyl 5' thioadenosine phosphorylase, S-methyl-5''-thioadenosine phosphorylase

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (7)

Enlarge Image

Figure 1: Western Blot - Anti-MTAP Antibody [ARC2872] (A305896)

Western blot analysis of extracts of various cell lines, using Anti-MTAP Antibody [ARC2872] (A305896) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

Enlarge Image

Figure 2: Immunohistochemistry - Anti-MTAP Antibody [ARC2872] (A305896)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-MTAP Antibody [ARC2872] (A305896) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Enlarge Image

Figure 3: Immunohistochemistry - Anti-MTAP Antibody [ARC2872] (A305896)

Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-MTAP Antibody [ARC2872] (A305896) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Enlarge Image

Figure 4: Immunofluorescence - Anti-MTAP Antibody [ARC2872] (A305896)

Immunofluorescence analysis of HeLa cells using Anti-MTAP Antibody [ARC2872] (A305896) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).