Anti-mTOR (phospho Ser2448) Antibody [ARC0094] (A308866)

Name: Anti-mTOR (phospho Ser2448) Antibody [ARC0094]
Brand: Antibodies.com
SKU: A308866
Price: 540.0 USD
Availability: InStock

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$540

Rabbit monoclonal [ARC0094] antibody to mTOR (phospho Ser2448) for WB.

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Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-mTOR (phospho Ser2448) Antibody [ARC0094]

Description

Rabbit monoclonal [ARC0094] antibody to mTOR (phospho Ser2448).

Applications

Dilutions

WB: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S2448 of human mTOR (P42345).

Sequence

TDSYS

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0094

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

289 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1), FK506 binding protein 12 rapamycin associated protein 1, FK506 binding protein 12 rapamycin associated protein 2, FK506 binding protein 12 rapamycin complex associated protein 1, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP rapamycin associated protein, FKBP12 rapamycin complex associated protein, FKBP12-rapamycin complex-associated protein, FKBP12-rapamycin complex-associated protein 1, FLJ44809, FRAP, FRAP1, FRAP2, Mammalian target of rapamycin, Mechanistic target of rapamycin, MTOR_HUMAN, OTTHUMP00000001983, RAFT1, Rapamycin and FKBP12 target 1, Rapamycin associated protein FRAP2, Rapamycin target protein, Rapamycin target protein 1, RAPT1, Serine/threonine-protein kinase mTOR

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-mTOR (phospho Ser2448) Antibody [ARC0094] (A308866)

Western blot analysis of extracts of 293T cells, using Anti-mTOR (phospho Ser2448) Antibody [ARC0094] (A308866) at 1:1,000 dilution. 293T cells were treated by 10% FBS at 37°C for 30 minutes after serum-starvation overnight or treated by IGF-1 (50 ng/ml) at 37°C for 5 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-mTOR (phospho Ser2448) Antibody [ARC0094] (A308866)

Western blot analysis of extracts of 293T cells, using Anti-mTOR (phospho Ser2448) Antibody [ARC0094] (A308866) at 1:1,000 dilution. 293T cells were treated by IGF-1 (50 ng/ml) at 37°C for 5 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 3: Western Blot - Anti-mTOR (phospho Ser2448) Antibody [ARC0094] (A308866)

Western blot analysis of extracts of NIH/3T3 cells, using Anti-mTOR (phospho Ser2448) Antibody [ARC0094] (A308866) at 1:1,000 dilution. NIH/3T3 cells were treated by EGF (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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