Anti-Muscle Actin Antibody [HHF35] (A250877)

Anti-Muscle Actin Antibody [HHF35]

Mouse monoclonal [HHF35] antibody to Muscle Actin.

This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).

Flow Cytometry, IF, IHC-P

Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, IHC-P: 0.25-0.5 µg/ml

Human, Rabbit, Feline, Canine, Mouse, Rat, Chicken

SDS extract of human myocardium.

Mouse

Monoclonal

HHF35

IgG1

Unconjugated

Protein A/G chromatography.

200 µg/ml

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-Muscle Actin Antibody [HHF35] - BSA and Azide free (A254057).

a actin, ACTA, ACTA1, ACTA2, ACTC, ACTC1, Actin, ACTSA, ACTS_HUMAN, Alpha 2 actin, alpha skeletal muscle, Alpha-actin-1, Cardiac muscle alpha actin 1, Skeletal muscle alpha actin 1