Anti-MyoD1 Antibody [5.8A] (A277720)

Anti-MyoD1 Antibody [5.8A]

Mouse monoclonal [5.8A] antibody to MyoD1.

This antibody recognizes a phosphor-protein of 45kDa, identified as MyoD1. The epitope of this MAb maps between amino acid 180-189 in the C-terminal of mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm s tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing s sarcomas and alveolar soft part sarcomas.

Flow Cytometry, IF, IHC-P

Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, IHC-P: 1-2 µg/ml

Human, Mouse, Rat, Chicken

Recombinant full-length mouse MyoD1 protein.

Mouse

Monoclonal

5.8A

IgG1

kappa

Protein A/G chromatography.

200 µg/ml

45 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-MyoD1 Antibody [5.8A] - BSA and Azide free (A278308).

bHLHc1, Class C basic helix-loop-helix protein 1, MYF 3, Myf-3, MYF3, Myoblast determination protein 1, MYOD, Myod 1, MYOD1_HUMAN, Myogenic differentiation 1, Myogenic factor 3, Myogenic factor MYF 3, Myogenin D1, PUM