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Anti-NCAM1 Antibody (A12624)

Western Blot - Anti-NCAM1 Antibody (A12624) - Antibodies.com Immunohistochemistry - Anti-NCAM1 Antibody (A12624) - Antibodies.com Immunohistochemistry - Anti-NCAM1 Antibody (A12624) - Antibodies.com
$435
Product Datasheet

Rabbit polyclonal antibody to NCAM1 for WB, IHC and ICC/IF.

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5-8 Business Days

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Rabbit polyclonal antibody to CD56 / NCAM for ELISA, IHC, IP and WB.
Name
Anti-NCAM1 Antibody
Description
Rabbit polyclonal antibody to NCAM1.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:100
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 759-858 of human NCAM1 / CD56 (NP_851996.2).
Sequence
LCGKAGPGAKGKDMEEGKAAFSKDESKEPIVEVRTEEERTPNHDGGKHTEPNETTPLTEPEKGPVEAKPECQETETKPAPAEVKTVPNDATQTKENESKA
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
120 kDa / 140 kDa / 180 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
antigen MSK39 identified by monoclonal, antigen recognized by monoclonal, CD56, cell adhesion molecule, neural, 1, MSK 39, MSK39, N-CAM-1, NCAM, NCAM 1, NCAM C, NCAM-1, NCAM1_HUMAN, NCAMC, Neural cell adhesion molecule 1, Neural cell adhesion molecule NCAM, OTTHUMP00000235666
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-NCAM1 Antibody (A12624) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-NCAM1 Antibody (A12624)
Western blot analysis of extracts of mouse brain, using Anti-NCAM1 Antibody (A12624). The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST.
Immunohistochemistry - Anti-NCAM1 Antibody (A12624) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-NCAM1 Antibody (A12624)
Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-NCAM1 Antibody (A12624) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-NCAM1 Antibody (A12624) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-NCAM1 Antibody (A12624)
Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-NCAM1 Antibody (A12624) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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