Anti-NCAM1 Antibody (A15971)

Name: Anti-NCAM1 Antibody
Brand: Antibodies.com
SKU: A15971
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to NCAM1 for WB, IHC, ICC/IF and IP.

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$40 to United States

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5-8 Business Days

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Schwann Cell Marker

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Specifications

Name

Anti-NCAM1 Antibody

Description

Rabbit polyclonal antibody to NCAM1.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 350-627 of human NCAM1 / CD56 (NP_851996.2).

Sequence

SEEKASWTRPEKQETLDGHMVVRSHARVSSLTLKSIQYTDAGEYICTASNTIGQDSQSMYLEVQYAPKLQGPVAVYTWEGNQVNITCEVFAYPSATISWFRDGQLLPSSNYSNIKIYNTPSASYLEVTPDSENDFGNYNCTAVNRIGQESLEFILVQADTPSSPSIDQVEPYSSTAQVQFDEPEATGGVPILKYKAEWRAVGEEVWHSKWYDAKEASMEGIVTIVGLKPETTYAVRLAALNGKGLGEISAASEFKTQPVQGEPSAPKLEGQMGEDGNS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

120 - 220 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

antigen MSK39 identified by monoclonal, antigen recognized by monoclonal, CD56, cell adhesion molecule, neural, 1, MSK 39, MSK39, N-CAM-1, NCAM, NCAM 1, NCAM C, NCAM-1, NCAM1_HUMAN, NCAMC, Neural cell adhesion molecule 1, Neural cell adhesion molecule NCAM, OTTHUMP00000235666

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-NCAM1 Antibody (A15971)

Western blot analysis of extracts of various cell lines, using Anti-NCAM1 Antibody (A15971) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Western Blot - Anti-NCAM1 Antibody (A15971)

Western blot analysis of extracts of SH-SY5Y cells, using Anti-NCAM1 Antibody (A15971) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 3: Immunohistochemistry - Anti-NCAM1 Antibody (A15971)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-NCAM1 Antibody (A15971) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-NCAM1 Antibody (A15971)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-NCAM1 Antibody (A15971) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.