Anti-Nephrin Antibody [ARC59568] (A309419)

Name: Anti-Nephrin Antibody [ARC59568]
Brand: Antibodies.com
SKU: A309419

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This product is discontinued

Anti-Nephrin Antibody [ARC59568] (A309419) has been discontinued and is no longer available.

View all Anti-Nephrin Antibodies.

Specifications

Name

Anti-Nephrin Antibody [ARC59568]

Description

Rabbit monoclonal [ARC59568] antibody to Nephrin.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:100-1:500

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human Nephrin.

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC59568

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

202 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CNF, Nephrosis 1 congenital Finnish type, Nephrosis 1, congenital, Finnish type (nephrin), NPHN, NPHN_HUMAN, NPHS 1, Nphs1, Renal glomerulus specific cell adhesion receptor, Renal glomerulus-specific cell adhesion receptor

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-Nephrin Antibody [ARC59568] (A309419)

Western blot analysis of various lysates, using Anti-Nephrin Antibody [ARC59568] (A309419) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.

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Figure 2: Immunohistochemistry - Anti-Nephrin Antibody [ARC59568] (A309419)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-Nephrin Antibody [ARC59568] (A309419) at a dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Nephrin Antibody [ARC59568] (A309419)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-Nephrin Antibody [ARC59568] (A309419) at a dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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