Anti-Nestin Antibody [Rat-401 (4D4)] (A281649)

Anti-Nestin Antibody [Rat-401 (4D4)]

Mouse monoclonal [Rat-401 (4D4)] antibody to Nestin.

This antibody recognises rat nestin, a large intermediate filament protein transiently expressed in embryonic glian cells. It is predominately expressed in stem cells of the developing nervous system. Terminal differentiation is associated with a loss of nestin expression. Nestin expression has also been noted in other embryonic tissues, also in most Glioblastoma multiformes and many melanomas.

WB, IHC-P, IF, IHC-Fr

IHC-Fr: 1:40 - 1:400, IHC-P: 1:40 - 1:400, We recommend perfusing tissues with 4% paraformaldehyde at pH 7.4 for light microscopy or with either 4% paraformaldehyde at pH 10.0 or 4% paraformaldehyde with 0.1% glutaraldehyde at pH 7.4 for EM.For ICC we recommend using cells fixed in 4% paraformaldehyde buffered with 50 mM sodium borate at pH 9.5. Mouse anti Rat Nestin antibody, clone Rat-401 reacts with a band at 200-220 kDa in reducing gels of newborn rat or mouse cell extracts.For WB it is recommended that samples should be boiled in 4 volumes of 125 mM Tris, pH 6.8, 10% 2-mercaptoethanol, 10% glycerol and 4.6% SDS. Membranes should be blocked with milk or BSA. 5% PAGE gels are suggested.

Rat, Mouse

This antibody does not cross react with Human

Nestin purified from embryonic rat spinal cord.

Mouse

Monoclonal

Rat-401 (4D4)

IgG1

Protein A affinity chromatography of tissue culture supernatant.

1 mg/ml

200-220 kDa in reducing gels of newborn rat or mouse cell extracts.

Liquid

Supplied in TRIS buffered glycine with 0.05% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Rat Nestin antibody, clone Rat-401 recognizes rat nestin, a large intermediate filament protein transiently expressed in embryonic glian cells (Hockfield and McKay 1985). It is predominately expressed in stem cells of the developing nervous system. Terminal differentiation is associated with a loss of nestin expression. Nestin expression has also been noted in other embryonic tissues, also in most Glioblastoma multiformes and many melanomas.

ESTM 46, FLJ 21841, FLJ21841, Intermediate filament protein, Nbla00170, nes, NEST_HUMAN