Figure 1: Western Blot - Anti-Neurofilament heavy polypeptide Antibody [ARC0494] (A305353)
Western blot analysis of extracts of various cell lines, using Anti-Neurofilament heavy polypeptide Antibody [ARC0494] (A305353) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Figure 2: Immunohistochemistry - Anti-Neurofilament heavy polypeptide Antibody [ARC0494] (A305353)
Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-Neurofilament heavy polypeptide Antibody [ARC0494] (A305353) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Figure 3: Immunohistochemistry - Anti-Neurofilament heavy polypeptide Antibody [ARC0494] (A305353)
Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-Neurofilament heavy polypeptide Antibody [ARC0494] (A305353) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Figure 4: Immunofluorescence - Anti-Neurofilament heavy polypeptide Antibody [ARC0494] (A305353)
Immunofluorescence analysis of Neuro-2a using Anti-Neurofilament heavy polypeptide Antibody [ARC0494] (A305353) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Figure 5: Western Blot - Anti-Neurofilament heavy polypeptide Antibody [ARC0494] (A305353)
Immunoprecipitation analysis of 600µg extracts of rat brain cells using 3µg of Anti-Neurofilament heavy polypeptide Antibody [ARC0494] (A305353). This Western blot was performed on the immunoprecipitate using Anti-Neurofilament heavy polypeptide Antibody [ARC0494] (A305353) at a dilution of 1:500.
Figure 6: Western Blot - Anti-Neurofilament heavy polypeptide Antibody [ARC0494] (A305353)
Immunoprecipitation analysis of 300µg extracts of PC-3 cells using 3µg of Anti-Neurofilament heavy polypeptide Antibody [ARC0494] (A305353). This Western blot was performed on the immunoprecipitate using Anti-Neurofilament heavy polypeptide Antibody [ARC0494] (A305353) at a dilution of 1:500.
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