Anti-NRF2 Antibody (A304864)

Name: Anti-NRF2 Antibody
Brand: Antibodies.com
SKU: A304864
Price: 500.0 USD
Availability: InStock

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$500

Rabbit polyclonal antibody to NRF2 for WB, ICC/IF and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-NRF2 Antibody

Description

Rabbit polyclonal antibody to NRF2.

Applications

WB, ICC/IF, IHC

Dilutions

WB: 1:1,000, ICC/IF: 1:100, IHC: 1:50

Reactivity

Human

Immunogen

Synthetic peptide from the mid-protein of Human NRF2.

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Peptide affinity purification.

Concentration

1 mg/ml

Molecular Weight

~67.8 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

erythroid derived 2, HEBP1, like 2, NF E2 related factor 2, NF-E2-related factor 2, NF2L2_HUMAN, NFE2 related factor 2, NFE2-related factor 2, Nfe2l2, Nrf 2, Nuclear factor, Nuclear factor (erythroid derived 2) like 2, nuclear factor erythroid 2 like 2, Nuclear factor erythroid 2 related factor 2, Nuclear factor erythroid 2-related factor 2, Nuclear factor erythroid derived 2 like 2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Immunohistochemistry - Anti-NRF2 Antibody (A304864)

Immunohistochemistry analysis of human kidney, fixed in formalin and paraffin-embedded. The Primary Antibody used was Anti-NRF2 Antibody (A304864) at 1:50 for 30 minutes at room temperature. Counterstain: Hematoxylin. Magnification: 20X.

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Figure 2: Western Blot - Anti-NRF2 Antibody (A304864)

Western blot analysis of human Cervical cancer cell line (HeLa) lysate showing detection of ~67.8 kDa NRF2 protein using Anti-NRF2 Antibody (A304864) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: ~67.8 kDa.

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Figure 3: Immunofluorescence - Anti-NRF2 Antibody (A304864)

Immunocytochemistry/Immunofluorescence analysis of human colon carcinoma cell line (RKO), fixed in 4% formaldehyde for 15 min at room temperature, using Anti-NRF2 Antibody (A304864), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Cytoplasm, Cytosol, Nucleus. Magnification: 60X.(A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) NRF2 Antibody. (D) Composite.

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