Anti-Nrf2 Antibody [ARC0806] (A309426)

Name: Anti-Nrf2 Antibody [ARC0806]
Brand: Antibodies.com
SKU: A309426

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This product is discontinued

Anti-Nrf2 Antibody [ARC0806] (A309426) has been discontinued and is no longer available.

View all Anti-Nrf2 Antibodies.

Specifications

Name

Anti-Nrf2 Antibody [ARC0806]

Description

Rabbit monoclonal [ARC0806] antibody to Nrf2.

Applications

WB, ICC/IF

Dilutions

WB: 1:100-1:500, ICC/IF: 1:50-1:200

Reactivity

Human

Immunogen

A synthesized peptide derived from human NRF2.

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0806

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

97 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

erythroid derived 2, HEBP1, like 2, NF E2 related factor 2, NF-E2-related factor 2, NF2L2_HUMAN, NFE2 related factor 2, NFE2-related factor 2, Nfe2l2, Nrf 2, Nuclear factor, Nuclear factor (erythroid derived 2) like 2, nuclear factor erythroid 2 like 2, Nuclear factor erythroid 2 related factor 2, Nuclear factor erythroid 2-related factor 2, Nuclear factor erythroid derived 2 like 2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-Nrf2 Antibody [ARC0806] (A309426)

Western blot analysis of extracts of HeLa cells, using Anti-Nrf2 Antibody [ARC0806] (A309426) at 1:500 dilution. HeLa cells were treated by MG132(50 µM) at 37°C for 90 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 2: Western Blot - Anti-Nrf2 Antibody [ARC0806] (A309426)

Western blot analysis of extracts from wild type(WT) and NRF2 knockout (KO) HeLa cells, using Anti-Nrf2 Antibody [ARC0806] (A309426) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 3: Immunofluorescence - Anti-Nrf2 Antibody [ARC0806] (A309426)

Immunofluorescence analysis of HepG2 using Anti-Nrf2 Antibody [ARC0806] (A309426) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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