Anti-p21 Antibody [ARC51040 + ARC52072] (A309680)

Name: Anti-p21 Antibody [ARC51040 + ARC52072]
Brand: Antibodies.com
SKU: A309680

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This product is discontinued

Anti-p21 Antibody [ARC51040 + ARC52072] (A309680) has been discontinued and is no longer available.

View all Anti-p21 Antibodies.

Specifications

Name

Anti-p21 Antibody [ARC51040 + ARC52072]

Description

Rabbit Oligoclonal [ARC51040 + ARC52072] antibody to p21.

Applications

WB, ICC/IF

Dilutions

WB: 1:100-1:500, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

Recombinant protein of human/mouse CDKN1A/p21.

Host

Rabbit

Clonality

Oligoclonal

Clone ID

ARC51040 + ARC52072

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CAP20, CDK-interacting protein 1, CDKI, CDKN1, Cdkn1a, CDN1A_HUMAN, CIP1, Cyclin Dependent Kinase Inhibitor 1A, Cyclin-dependent kinase inhibitor 1, Cyclin-dependent kinase inhibitor 1A (P21), Cyclin-dependent kinase inhibitor 1A (p21, Cip1), DNA Synthesis Inhibitor, MDA-6, MDA6, Melanoma differentiation-associated protein, Melanoma differentiation-associated protein 6, P21 protein, p21CIP1, p21Cip1/Waf1, p21WAF, PIC1, SDI1, SLC12A9, WAF1, Wild type p53 activated fragment 1, Wild type p53 activated fragment 1 (WAF1), Wildtype p53-activated fragment 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-p21 Antibody [ARC51040 + ARC52072] (A309680)

Western blot analysis of RAW264. 7, using Anti-p21 Antibody [ARC51040 + ARC52072] (A309680) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Western Blot - Anti-p21 Antibody [ARC51040 + ARC52072] (A309680)

Western blot analysis of various lysates, using Anti-p21 Antibody [ARC51040 + ARC52072] (A309680) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 3: Western Blot - Anti-p21 Antibody [ARC51040 + ARC52072] (A309680)

Western blot analysis of extracts from wild type(WT) and CDKN1A/p21 knockout (KO) 293T(KO) cells, using Anti-p21 Antibody [ARC51040 + ARC52072] (A309680) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 4: Immunofluorescence - Anti-p21 Antibody [ARC51040 + ARC52072] (A309680)

Immunofluorescence analysis of NIH/3T3 using Anti-p21 Antibody [ARC51040 + ARC52072] (A309680) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).

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