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Anti-p53 Antibody [BP53-12] (A86031)

Immunocytochemistry - Anti-p53 Antibody [BP53-12] (A86030) - Antibodies.com
$195
Product Datasheet

Mouse monoclonal [BP53-12] antibody to p53 for Flow Cytometry, IP, WB, IHC-P, ICC and ELISA.

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Alternative Formats and ConjugatesAlternative Formats

Freight Charges
$40 to United States
Lead Time
4-6 Business Days

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+1 (314) 370-6046
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Name
Anti-p53 Antibody [BP53-12]
Description
Mouse monoclonal [BP53-12] antibody to p53.
Specificity
This antibody recognizes a defined epitope, amino acids 16-25, on human p53, a 50 kDa tumour suppressor found in increased amounts in a wide variety of transformed cells; it is frequently mutated or inactivated in many types of cancer.
Applications
Flow Cytometry, IP, WB, IHC-P, ICC, ELISA
Dilutions
WB: 1-2 µg/ml, overnight at 4°C; Positive Control: RAMOS human lymphoma cell line, Non-reducing conditions. SDS-PAGE (12% separating gel), IHC: 5-10 µg/ml, Flow Cytometry: 1-4 µg/ml; Intracellular staining.
Reactivity
Human, Non-Human Primates
Immunogen
Bacterially expressed full length wild-type p53 protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
BP53-12
Isotype
IgG2a
Conjugate

Unconjugated

Purification
Purified by differential precipitation and solid-phase chromatography.
Concentration
1 mg/ml
Predicted MW
50 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Antigen NY-CO-13, BCC7, Cellular tumor antigen p53, FLJ92943, LFS1, Mutant tumor protein 53, p53 tumor suppressor, P53_HUMAN, Phosphoprotein p53, Tp53, Transformation related protein 53, TRP53, Tumor protein 53, Tumor protein p53, Tumor suppressor p53
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Immunocytochemistry - Anti-p53 Antibody [BP53-12] (A86030) - Antibodies.comEnlarge Image
Figure 1: Immunocytochemistry - Anti-p53 Antibody [BP53-12] (A86031)
Confocal microscopy of human HeLa cells using Anti-p53 Antibody (A86031). The expression of p53 protein was enhanced by intercalating reagent. Cells were fixed and permeabilized before incubation with Anti-p53 Antibody (A86031). Photo provided by Dr. Hodny, Inst. of Experimental Medicine, Prague, Czech Republic.
Publishing research using Anti-p53 Antibody [BP53-12] (A86031)? Please let us know so that we can list the citation on this page.

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