Anti-p53 Antibody [DO-11] (A280265)

Anti-p53 Antibody [DO-11]

Mouse monoclonal [DO-11] antibody to p53.

This antibody recognises the human Cellular tumor antigen p53, also known as p53 tumor suppressor protein or NY-CO-13. p53 is a 393 amino acid ~53kDa cytoplasmic/ nuclear protein up-regulated in response to DNA damage and is found in a wide variety of transformed cells (UniProt: P04637).DO-11 binds to an epitope within the central region of p53 between amino acids 181 - 190 defining a cryptic epitope exposed in unfolded/ denatured p53. Nine isoforms of human p53 are produced by alternative splicing and promotor usage, the epitope recognized by clone DO-11 is present in all isoforms. recognises mutant forms of p53 and has been used successfully for detection of p53 by western blotting.

WB, IHC-P, IHC-Fr, IP

IHC-P 1: 1:1,000, WB: 1:200 - 1:2,000

Human

Recombinant human p53.

Mouse

Monoclonal

DO-11

IgG1

Unconjugated

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human p53 antibody, clone DO-11 recognizes the human Cellular tumor antigen p53, also known as p53 tumor suppressor protein or NY-CO-13. p53 is a 393 amino acid ~53kDa cytoplasmic/ nuclear protein up-regulated in response to DNA damage and is found in a wide variety of transformed cells (UniProt: P04637).DO-11 binds to an epitope within the central region of p53 between amino acids 181 - 190 defining a cryptic epitope exposed in unfolded/ denatured p53 (Shimitzu et al. 2006). Nine isoforms of human p53 are produced by alternative splicing and promotor usage, the epitope recognized by clone DO-11 is present in all isoforms. Mouse anti Human p53 antibody, clone DO-11 recognizes mutant forms of p53 (Warnock et al. 2011) and has been used successfully for detection of p53 by western blotting (Wei et al. 2012).

Antigen NY-CO-13, BCC7, Cellular tumor antigen p53, FLJ92943, LFS1, Mutant tumor protein 53, p53 tumor suppressor, P53_HUMAN, Phosphoprotein p53, Tp53, Transformation related protein 53, TRP53, Tumor protein 53, Tumor protein p53, Tumor suppressor p53