Western Blot - Anti-p53 (phospho Ser9) Antibody [ARC1529] (A305625)
Western blot analysis of extracts of NIH/3T3 cells, using Anti-p53 (phospho Ser9) Antibody [ARC1529] (A305625) at 1:1,000 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.
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