Anti-Parvalbumin Antibody [C12] (A305173)

Immunohistochemistry analysis of mouse thyroid. The Primary Antibody used was Anti-Parvalbumin Antibody [C12] (A305173) at 1:100 for Overnight at 4°C, then 30 minutes at 37°C. The secondary antibody used was Goat Anti-Mouse IgG (H+L): FITC for 45 minutes at 37°C. Counterstain: DAPI for 3 min at room temperature. Magnification: 5X.

Western blot analysis of rat brain showing detection of Parvalbumin protein using Anti-Parvalbumin Antibody [C12] (A305173) at 1:500 for 2 hours at room temperature with shaking. Load: 15 ug. The secondary antibody used was Goat anti-mouse IgG:HRP at 1:4,000 for 1 hour at room temperature with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 minutes in room temperature.

Immunocytochemistry/Immunofluorescence analysis of human undifferentiated SH-SY5Y, using Anti-Parvalbumin Antibody [C12] (A305173), at 1:250. The secondary antibody used was AlexaFluor 488. Counterstain: phalloidin (Alexa 647, red), beta tubulin (Anti-beta III Tubulin Ab, Alexa 555, magenta) Hoechst (blue).(A) Phalloidin (B) Anti-beta III Tubulin Ab. (C) Parvalbumin Antibody. (D) Hoechst (E) Composite.

Immunohistochemistry analysis of human thyroid cancer. The Primary Antibody used was Anti-Parvalbumin Antibody [C12] (A305173) at 1:100 for Overnight at 4°C, then 30 minutes at 37°C. The secondary antibody used was Goat Anti-Mouse IgG (H+L): FITC for 45 minutes at 37°C. Counterstain: DAPI for 3 min at room temperature. Magnification: 5X.