Flow Cytometry: 1-5 µg/ml; Intracellular staining, WB: A small splice variant of PCLO can be analyzed by Western blotting, however, the whole protein is too large (over 400 kDa) to be successfully processed by SDS PAAGE.

Reactivity

Human

Immunogen

Human recombinant PCLO protein.

Host

Mouse

Clonality

Monoclonal

Clone ID

PCLO-01

Isotype

IgG1

Conjugate

Unconjugated

Purification

Protein A chromatography.

Concentration

1 mg/ml

Predicted MW

400 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

ACZ, Aczonin, Brain-derived HLMN protein, DKFZp779G1236, KIAA0559, Multidomain presynaptic cytomatrix protein, PCLO, PCLO_HUMAN, Piccolo (presynaptic cytomatrix protein), Piccolo, mouse, homolog of, Pico, Presynaptic cytomatrix protein, Protein piccolo

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (1)

Enlarge Image

Figure 1: Western Blot - Anti-Piccolo Antibody [PCLO-01] (A86487)

Western blot of human REH erythroid cell line lysate (1% laurylmaltoside); non-reduced sample, immunostained by Anti-PCLO Antibody (A86487) and Goat Anti-Mouse IgG Antibody (H+L) (HRP). The zone evidently corresponds to a small splice variant of the PCLO protein present in this type of cell (see Uniprot database, Genome Res. 14:2121-2127(2004)); the major form (over 500 kDa) expressed in neurons is too large for analysis by Western blotting.

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