Anti-PCNA Antibody [ARC51324] (A305487)

Name: Anti-PCNA Antibody [ARC51324]
Brand: Antibodies.com
SKU: A305487
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC51324] antibody to PCNA for WB, IHC, ICC/IF and IP.

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$40 to United States

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5-8 Business Days

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Nuclear Loading Control

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Specifications

Name

Anti-PCNA Antibody [ARC51324]

Description

Rabbit monoclonal [ARC51324] antibody to PCNA.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:2,000-1:9,000, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat, African green monkey

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 162-261 of human PCNA (NP_002583.1).

Sequence

CAKDGVKFSASGELGNGNIKLSQTSNVDKEEEAVTIEMNEPVQLTFALRYLNFFTKATPLSSTVTLSMSADVPLVVEYKIADMGHLKYYLAPKIEDEEGS

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC51324

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

35 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ATLD2, cb16, Cyclin, DNA polymerase delta auxiliary protein, etID36690.10, fa28e03, fb36g03, HGCN8729, MGC8367, Mutagen-sensitive 209 protein, OTTHUMP00000030189, OTTHUMP00000030190, Pcna/cyclin, PCNAR, PCNA_HUMAN, Polymerase delta accessory protein, Proliferating cell nuclear antigen, wu:fa28e03, wu:fb36g03

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (9)

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Figure 1: Western Blot - Anti-PCNA Antibody [ARC51324] (A305487)

Western blot analysis of various lysates, using Anti-PCNA Antibody [ARC51324] (A305487) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Immunohistochemistry - Anti-PCNA Antibody [ARC51324] (A305487)

Immunohistochemistry analysis of paraffin-embedded human esophagus using Anti-PCNA Antibody [ARC51324] (A305487) at a dilution of 1:8,000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-PCNA Antibody [ARC51324] (A305487)

Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-PCNA Antibody [ARC51324] (A305487) at a dilution of 1:8,000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-PCNA Antibody [ARC51324] (A305487)

Immunohistochemistry analysis of paraffin-embedded mouse esophagus using Anti-PCNA Antibody [ARC51324] (A305487) at a dilution of 1:8,000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.