Anti-PDPK1 (phospho Ser241) Antibody (A10962)

Name: Anti-PDPK1 (phospho Ser241) Antibody
Brand: Antibodies.com
SKU: A10962
Price: 435.0 USD
Availability: InStock

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$435

Rabbit polyclonal antibody to PDPK1 (phospho Ser241) for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-PDPK1 (phospho Ser241) Antibody

Description

Rabbit polyclonal antibody to PDPK1 (phospho Ser241).

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S241 of human PDK1/PDPK1 (NP_002604.1).

Sequence

ANSFV

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

60 - 70 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

3 phosphoinositide dependent protein kinase 1, 3-phosphoinositide-dependent protein kinase 1, hPDK 1, hPDK1, MGC20087, MGC35290, OTTHUMP00000159109, OTTHUMP00000159110, OTTHUMP00000174525, PDK1, PDPK1_HUMAN, PDPK2, PDPK2P, PkB kinase, PkB kinase like gene 1, PkB like 1, PRO0461, Protein kinase

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-PDPK1 (phospho Ser241) Antibody (A10962)

Western blot analysis of extracts of NIH/3T3 cells, using Anti-PDPK1 (phospho Ser241) Antibody (A10962) at 1:1,000 dilution. NIH/3T3 cells were treated by PDGF (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 2: Immunofluorescence - Anti-PDPK1 (phospho Ser241) Antibody (A10962)

Immunofluorescence analysis of C6 cells using Anti-PDPK1 (phospho Ser241) Antibody (A10962) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 3: Immunofluorescence - Anti-PDPK1 (phospho Ser241) Antibody (A10962)

Immunofluorescence analysis of NIH-3T3 cells using Anti-PDPK1 (phospho Ser241) Antibody (A10962) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-PDPK1 (phospho Ser241) Antibody (A10962)

Immunofluorescence analysis of U-2 OS cells using Anti-PDPK1 (phospho Ser241) Antibody (A10962) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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