Anti-PGAM5 Antibody [ARC53895] (A305956)

Name: Anti-PGAM5 Antibody [ARC53895]
Brand: Antibodies.com
SKU: A305956

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This product is discontinued

Anti-PGAM5 Antibody [ARC53895] (A305956) has been discontinued and is no longer available.

View all Anti-PGAM5 Antibodies.

Specifications

Name

Anti-PGAM5 Antibody [ARC53895]

Description

Rabbit monoclonal [ARC53895] antibody to PGAM5.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:2,000-1:20,000, IHC: 1:50-1:200, ICC/IF: 1:100-1:500

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 50-146 of human PGAM5 (NP_001164014.1).

Sequence

GARPGPGVWDPNWDRREPLSLINVRKRNVESGEEELASKLDHYKAKATRHIFLIRHSQYHVDGSLEKDRTLTPLGREQAELTGLRLASLGLKFNKIV

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC53895

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

28 kDa / 32 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Bcl-XL-binding protein v68, BXLBv68, MGC5352, mitochondrial, PGAM5_HUMAN, Phosphoglycerate mutase family member 5, Serine/threonine protein phosphatase PGAM5 mitochondrial, Serine/threonine-protein phosphatase PGAM5

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-PGAM5 Antibody [ARC53895] (A305956)

Western blot analysis of various lysates, using Anti-PGAM5 Antibody [ARC53895] (A305956) at 1:20,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Western Blot - Anti-PGAM5 Antibody [ARC53895] (A305956)

Western blot analysis of Rat kidney, using Anti-PGAM5 Antibody [ARC53895] (A305956) at 1:20,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 3: Immunohistochemistry - Anti-PGAM5 Antibody [ARC53895] (A305956)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-PGAM5 Antibody [ARC53895] (A305956) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-PGAM5 Antibody [ARC53895] (A305956)

Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using Anti-PGAM5 Antibody [ARC53895] (A305956) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.