Recombinant Anti-Phosphothreonine Antibody [RM111] (A121324)

Name: Anti-Phosphothreonine Antibody [RM111]
Brand: Antibodies.com
SKU: A121324
Price: 535.0 USD
Availability: InStock

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$535

Recombinant rabbit monoclonal [RM111] antibody to Phosphothreonine for WB, IP, ChIP, ICC and IHC.

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Freight Charges

$40 to United States

Lead Time

4-6 Business Days

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Recombinant Antibody

Specifications

Name

Anti-Phosphothreonine Antibody [RM111]

Description

Recombinant rabbit monoclonal [RM111] antibody to Phosphothreonine.

Specificity

This antibody recognizes tyrosine-phosphorylated proteins.

Applications

WB, IP, ChIP, ICC, IHC

Dilutions

WB: 1:1,000 -1:5,000, IP: 1:200-1:1,000, ChIP: 1:200-1:1,000, ICC: 1:200-1:500, IHC: 1:200-1:500

Reactivity

All Species

Cross Reactivity

There is no cross reactivity with non-phosphorylated tyrosine, phosphoserine, and phosphorthreonine.

Immunogen

Phosphotyrosine conjugated to BSA.

Host

Rabbit

Clonality

Monoclonal

Clone ID

RM111

Isotype

IgG

Conjugate

Unconjugated

Purification

Protein A chromatography.

Concentration

1 mg/ml

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Immunocytochemistry - Anti-Phosphothreonine Antibody [RM111] (A121324)

Immunocytochemistry of serum-starved A431 cells non-treated or treated with EGF, using Anti-Phosphothreonine Antibody [RM111] at 1:500 dilution (followed by a PE conjugated secondary antibody, red) and DAPI (blue).

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Figure 2: Western Blot - Anti-Phosphothreonine Antibody [RM111] (A121324)

Western blot of serum-starved A431 cells non-treated or treated with EGF, using Anti-Phosphothreonine Antibody [RM111] at a 1:5,000 dilution. The blot was exposed to the film at different time points.

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Figure 3: Immunoprecipitation - Anti-Phosphothreonine Antibody [RM111] (A121324)

Immunoprecipitation of EGF-treated A431 cells by Anti-Phosphothreonine Antibody [RM111] at 1:1,000 dilution, was blotted with an Anti-EGFR Antibody. (1) Whole lysate control. (2) IP by rabbit IgG control. (3) IP by Anti-Phosphothreonine Antibody [RM111].

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Figure 4: Anti-Phosphothreonine Antibody [RM111] (A121324)

Luminex analysis of Anti-Phosphothreonine Antibody [RM111]’s reaction against phosphorylated or non-phosphorylated Serine, Threonine, and Tyrosine. Anti-Phosphothreonine Antibody [RM111] specifically reacts to phosphorylated Tyrosine.

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