Anti-PINK1 Antibody (A80580)

Name: Anti-PINK1 Antibody
Brand: Antibodies.com
SKU: A80580

Enlarge Image

This product is discontinued

Anti-PINK1 Antibody (A80580) has been discontinued and is no longer available.

View all Anti-PINK1 Antibodies.

Specifications

Name

Anti-PINK1 Antibody

Description

Rabbit polyclonal antibody to PINK1.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 282-581 of human PINK1 (NP_115785.1).

Sequence

TSSVPLLPGALVDYPDVLPSRLHPEGLGHGRTLFLVMKNYPCTLRQYLCVNTPSPRLAAMMLLQLLEGVDHLVQQGIAHRDLKSDNILVELDPDGCPWLVIADFGCCLADESIGLQLPFSSWYVDRGGNGCLMAPEVSTARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGKAHLESRSYQEAQLPALPESVPPDVRQLVRALLQREASKRPSARVAANVLHLSLWGEHILALKNLKLDKMVGWLLQQSAATLLANRLTEKCCVETKMKMLFLANLECETLCQAALLLCSWRAAL

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

50 kDa / 63 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

BRPK, FLJ27236, mitochondrial, PARK 6, PARK6, Phosphatase and Tensin Homolog, PINK 1, PINK1_HUMAN, Protein kinase BRPK, PTEN induced putative kinase 1, PTEN induced putative kinase protein 1, PTEN-induced putative kinase protein 1, Serine/threonine kinase PINK1 mitochondrial, Serine/threonine protein kinase PINK1 mitochondrial, Serine/threonine-protein kinase PINK1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

Enlarge Image

Figure 1: Western Blot - Anti-PINK1 Antibody (A80580)

Western blot analysis of extracts of Rat liver, using Anti-PINK1 Antibody (A80580) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

Enlarge Image

Figure 2: Western Blot - Anti-PINK1 Antibody (A80580)

Western blot analysis of extracts of various cell lines, using Anti-PINK1 Antibody (A80580) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

Enlarge Image

Figure 3: Immunofluorescence - Anti-PINK1 Antibody (A80580)

Immunofluorescence analysis of A549 cells using Anti-PINK1 Antibody (A80580) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).

Enlarge Image

Figure 4: Immunofluorescence - Anti-PINK1 Antibody (A80580)

Immunofluorescence analysis of NIH-3T3 cells using Anti-PINK1 Antibody (A80580) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).