Anti-PINK1 Antibody [S4-15] (A305003)

Name: Anti-PINK1 Antibody [S4-15]
Brand: Antibodies.com
SKU: A305003
Price: 500.0 USD
Availability: InStock

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$500

Mouse monoclonal [S4-15] antibody to PINK1 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-PINK1 Antibody [S4-15]

Description

Mouse monoclonal [S4-15] antibody to PINK1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein amino acids 112-496 (cytoplasmic C-terminus) of human PINK1. 82% identical to rat and 81% identical to mouse. >30% identity with DMPK

Host

Mouse

Clonality

Monoclonal

Clone ID

S4-15

Isotype

IgG1

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~50 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.1% Sodium Azide

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

BRPK, FLJ27236, mitochondrial, PARK 6, PARK6, Phosphatase and Tensin Homolog, PINK 1, PINK1_HUMAN, Protein kinase BRPK, PTEN induced putative kinase 1, PTEN induced putative kinase protein 1, PTEN-induced putative kinase protein 1, Serine/threonine kinase PINK1 mitochondrial, Serine/threonine protein kinase PINK1 mitochondrial, Serine/threonine-protein kinase PINK1

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-PINK1 Antibody [S4-15] (A305003)

Western blot analysis of rat brain showing detection of ~50 kDa PINK1 protein using Anti-PINK1 Antibody [S4-15] (A305003) at 1:200 for 16 hours at 4°C. Lane 1: Molecular Weight Ladder. Lane 2: rat Brain. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Mouse IgG: HRP at 1:1,000 for 1 hour room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: ~50 kDa.

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Figure 2: Immunofluorescence - Anti-PINK1 Antibody [S4-15] (A305003)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-PINK1 Antibody [S4-15] (A305003), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5,000 for 60 minutes room temperature, 5 minutes room temperature. Localization: Cytoplasm. Magnification: 60X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) PINK1 Antibody. (D) Composite.

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Figure 3: Immunofluorescence - Anti-PINK1 Antibody [S4-15] (A305003)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-PINK1 Antibody [S4-15] (A305003), at 1:50 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) PINK1 Antibody (D) Composite.

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