Anti-PKC alpha Antibody (A12587)

Name: Anti-PKC alpha Antibody
Brand: Antibodies.com
SKU: A12587
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to PKC alpha for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-PKC alpha Antibody

Description

Rabbit polyclonal antibody to PKC alpha.

Applications

WB, IHC

Dilutions

WB: 1:100-1:500, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 573-672 of human PKC alpha (NP_002728.2).

Sequence

MTKHPAKRLGCGPEGERDVREHAFFRRIDWEKLENREIQPPFKPKVCGKGAENFDKFFTRGQPVLTPPDQLVIANIDQSDFEGFSYVNPQFVHPILQSAV

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

80 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AAG6, Aging associated gene 6, aPKC, KPCA_HUMAN, PKC-A, PKC-alpha, PKCA, PRKACA, PRKCA, Protein Kinase C alpha, Protein kinase C alpha type

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-PKC alpha Antibody (A12587)

Western blot analysis of extracts of various cell lines, using Anti-PKC alpha Antibody (A12587) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Immunohistochemistry - Anti-PKC alpha Antibody (A12587)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-PKC alpha Antibody (A12587) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-PKC alpha Antibody (A12587)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-PKC alpha Antibody (A12587) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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