Western Blot - Anti-PKC alpha (phospho S226) Antibody (A11037)
Western blot analysis of extracts of 293 cells, using Anti-PKC alpha (phospho S226) Antibody (AP0559) at 1:1,000 dilution. 293T cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% BSA.
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