Anti-PLK1 Antibody [ARC52313] (A306405)

Name: Anti-PLK1 Antibody [ARC52313]
Brand: Antibodies.com
SKU: A306405
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC52313] antibody to PLK1 for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-PLK1 Antibody [ARC52313]

Description

Rabbit monoclonal [ARC52313] antibody to PLK1.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat, Monkey

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 272-495 of human PLK1 (NP_005021.2).

Sequence

KHINPVAASLIQKMLQTDPTARPTINELLNDEFFTSGYIPARLPITCLTIPPRFSIAPSSLDPSNRKPLTVLNKGLENPLPERPREKEEPVVRETGEVVDCHLSDMLQQLHSVNASKPSERGLVRQEEAEDPACIPIFWVSKWVDYSDKYGLGYQLCDNSVGVLFNDSTRLILYNDGDSLQYIERDGTESYLTVSSHPNSLMKKITLLKYFRNYMSEHLLKAGA

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC52313

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Cell cycle regulated protein kinase, PLK, PLK 1, PLK-1, PLK1_HUMAN, Polo like kinase 1, Polo-like kinase 1, Serine/threonine protein kinase 13, Serine/threonine protein kinase PLK1, Serine/threonine-protein kinase 13, Serine/threonine-protein kinase PLK1, STPK 13, STPK13

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (9)

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Figure 1: Western Blot - Anti-PLK1 Antibody [ARC52313] (A306405)

Western blot analysis of various lysates, using Anti-PLK1 Antibody [ARC52313] (A306405) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Western Blot - Anti-PLK1 Antibody [ARC52313] (A306405)

Western blot analysis of Mouse testis, using Anti-PLK1 Antibody [ARC52313] (A306405) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 3: Western Blot - Anti-PLK1 Antibody [ARC52313] (A306405)

Western blot analysis of Rat testis, using Anti-PLK1 Antibody [ARC52313] (A306405) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 120s.

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Figure 4: Immunohistochemistry - Anti-PLK1 Antibody [ARC52313] (A306405)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-PLK1 Antibody [ARC52313] (A306405) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.