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Anti-Podoplanin / gp36 Antibody (A305444)

Western Blot - Anti-Podoplanin / gp36 Antibody (A305444) - Antibodies.com Western Blot - Anti-Podoplanin / gp36 Antibody (A305444) - Antibodies.com Immunohistochemistry - Anti-Podoplanin / gp36 Antibody (A305444) - Antibodies.com Immunohistochemistry - Anti-Podoplanin / gp36 Antibody (A305444) - Antibodies.com Immunohistochemistry - Anti-Podoplanin / gp36 Antibody (A305444) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to Podoplanin / gp36 for WB and IHC.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com

Name
Anti-Podoplanin / gp36 Antibody
Description
Rabbit polyclonal antibody to Podoplanin / gp36.
Applications
WB, IHC
Dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 99-199 of human Podoplanin (NP_006465.3).
Sequence
ASTGQPEDDTETTGLEGGVAMPGAEDDVVTPGTSEDRYKSGLTTLVATSVNSVTGIRIEDLPTSESTVHAQEQSPSATASNVATSHSTEKVDGDTQTTVEK
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Aggrus, Glycoprotein 36, Glycoprotein 36 KD, gp 36, GP 38, GP 40, gp36, GP38, GP40, HT1A 1, HT1A1, hT1alpha 1, hT1alpha 2, hT1alpha1, hT1alpha2, Lung type I cell membrane associated glycoprotein, Lung type I cell membrane associated glycoprotein isoform a, Lung type I cell membrane associated glycoprotein T1A 2, OTS 8, OTS8, OTTHUMP00000009640, OTTHUMP00000044504, PA2.26, PA2.26 antigen, Pdpn, PDPN_HUMAN, Podoplanin, PSEC0003, PSEC0025, T1 alpha, T1 ALPHA GENE, T1-alpha, T1A, T1A 2, TI1A, TIA 2, TIA2
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Podoplanin / gp36 Antibody (A305444) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Podoplanin / gp36 Antibody (A305444)
Western blot analysis of U2OS, using Anti-Podoplanin / gp36 Antibody (A305444) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Western Blot - Anti-Podoplanin / gp36 Antibody (A305444) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-Podoplanin / gp36 Antibody (A305444)
Western blot analysis of various lysates, using Anti-Podoplanin / gp36 Antibody (A305444) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry - Anti-Podoplanin / gp36 Antibody (A305444) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-Podoplanin / gp36 Antibody (A305444)
Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-Podoplanin / gp36 Antibody (A305444) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-Podoplanin / gp36 Antibody (A305444) - Antibodies.comEnlarge Image
Figure 4: Immunohistochemistry - Anti-Podoplanin / gp36 Antibody (A305444)
Immunohistochemistry analysis of paraffin-embedded human lung using Anti-Podoplanin / gp36 Antibody (A305444) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Publishing research using Anti-Podoplanin / gp36 Antibody (A305444)? Please let us know so that we can list the citation on this page.

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