Rabbit polyclonal antibody to Progesterone Receptor (Phospho-Ser400)
Specificity
The antibody detects endogenous level of Progesterone Receptor only when phosphorylated at serine 400.
Applications
WB
Dilutions
Western blotting: 1:500~1:1000
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of serine 400 (A-R-S(p)-P-R) derived from Human Progesterone Receptor.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20˚C / 1 year
Synonyms
NR3C3, Nuclear receptor subfamily 3 group C member 3, PGR, PR
Western blot analysis of extracts from 293 cells treated with Heatshock using Phospho-Progesterone Receptor (Ser400) antibody #11992.The lane on the right is treated with the antigen-specific peptide.
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