Anti-PUMA Antibody (A304946)

Name: Anti-PUMA Antibody
Brand: Antibodies.com
SKU: A304946
Price: 500.0 USD
Availability: InStock

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$500

Rabbit polyclonal antibody to PUMA for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-PUMA Antibody

Description

Rabbit polyclonal antibody to PUMA.

Applications

WB, ICC/IF

Dilutions

WB: 1:1,000, ICC/IF: 1:100

Reactivity

Human, Mouse, Chicken

Immunogen

C-terminal amino acids of human PUMA.

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Protein A purification.

Concentration

1 mg/ml

Molecular Weight

~23 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

BBC 3, Bbc3, BBC3_HUMAN, BCL 2 binding component 3, Bcl-2-binding component 3, BCL2 binding component 3, JFY 1, JFY-1, JFY1, p53 up regulated modulator of apoptosis, p53 up-regulated modulator of apoptosis, p53 Upregulated Modulator of Apoptosis, PUMA alpha, PUMA/JFY1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Immunofluorescence - Anti-PUMA Antibody (A304946)

Immunocytochemistry/Immunofluorescence analysis of human K562 cells, using Anti-PUMA Antibody (A304946), at 1:100.

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Figure 2: Immunofluorescence - Anti-PUMA Antibody (A304946)

Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-PUMA Antibody (A304946), at 1:125 for 12 hours at 4°C. The secondary antibody used was R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Mitochondrion. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-PUMA (CT) Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

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Figure 3: Western Blot - Anti-PUMA Antibody (A304946)

Western blot analysis of Human, mouse K562 and 3T3 cell lysates showing detection of PUMA protein using Anti-PUMA Antibody (A304946) at 1:1,000.

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Figure 4: Immunofluorescence - Anti-PUMA Antibody (A304946)

Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-PUMA Antibody (A304946), at 1:125 for 12 hours at 4°C. The secondary antibody used was APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Mitochondrion. Magnification: 100x.(A) DAPI (blue) nuclear stain. (B) Anti-PUMA (CT) Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

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