Anti-PUMA Antibody [ARC0247] (A305311)

Name: Anti-PUMA Antibody [ARC0247]
Brand: Antibodies.com
SKU: A305311
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0247] antibody to PUMA for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-PUMA Antibody [ARC0247]

Description

Rabbit monoclonal [ARC0247] antibody to PUMA.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human PUMA (Q9BXH1).

Sequence

AEQHLESPVPSAPGALAGGPTQAAPGVRGEEEQWAREIGAQLRRMADDLNAQYERRRQEEQQRHRPSPWRVLYNLIMGLLPLPRGHRAPEMEPN

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0247

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

18 kDa / 23 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

BBC 3, Bbc3, BBC3_HUMAN, BCL 2 binding component 3, Bcl-2-binding component 3, BCL2 binding component 3, JFY 1, JFY-1, JFY1, p53 up regulated modulator of apoptosis, p53 up-regulated modulator of apoptosis, p53 Upregulated Modulator of Apoptosis, PUMA alpha, PUMA/JFY1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-PUMA Antibody [ARC0247] (A305311)

Western blot analysis of extracts of various cell lines, using Anti-PUMA Antibody [ARC0247] (A305311) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Immunohistochemistry - Anti-PUMA Antibody [ARC0247] (A305311)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-PUMA Antibody [ARC0247] (A305311) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-PUMA Antibody [ARC0247] (A305311)

Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using Anti-PUMA Antibody [ARC0247] (A305311) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-PUMA Antibody [ARC0247] (A305311)

Immunofluorescence analysis of C6 cells using Anti-PUMA Antibody [ARC0247] (A305311) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).