Recombinant Anti-Rb Antibody [rRB1/8060] (A315541)

$765

Recombinant mouse monoclonal [rRB1/8060] antibody to Rb for WB and IHC-P.

Alternative Formats and ConjugatesAlternative Formats

Freight Charges
Lead Time
4-6 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Anti-Rb Antibody [rRB1/8060]
Description
Recombinant mouse monoclonal [rRB1/8060] antibody to Rb.
Specificity
Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. Its epitope is localized between aa 703-772. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.
Applications
WB, IHC-P
Positive Control
HeLa, A431 or BT-20 cells. Human breast or colon carcinoma.
Dilutions
WB: 1-2 µg/ml, IHC: 1-2 µg/ml
Reactivity
Human, Mouse
Immunogen
Recombinant human Rb1 protein fragment of 283 amino acid residues.
Host
Mouse
Clonality
Monoclonal
Clone ID
rRB1/8060
Isotype
IgG1
Light Chains
kappa
Conjugate

Unconjugated

Purification
Protein A/G chromatography.
Concentration
200 µg/ml
Molecular Weight
105 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Exon 17 tumor GOS561 substitution mutation causes premature stop, GOS563 exon 17 substitution mutation causes premature stop, OSRC, Osteosarcoma, p105-Rb, P105RB, PP105, pp110, PPP1R130, pRb, Prepro retinoblastoma associated protein, Protein phosphatase 1 regulatory subunit 130, RB transcriptional corepressor 1, RB1, RB1 gene, RB_HUMAN, Retinoblastoma 1, Retinoblastoma suspectibility protein, Retinoblastoma-associated protein
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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